gsk j4  (MedChemExpress)

Journal: Medical Oncology (Northwood, London, England)

Article Title: Anti-tumor effects and mechanism of the histone demethylase inhibitor GSK-J4 in non-small cell lung cancer cells

doi: 10.1007/s12032-025-03185-3

Figure Lengend Snippet: GSK-J4 inhibits the migration and invasion of A549 and H1299 cells induced by TGFβ1. ( A ) Wound healing experiments. Control group, TGFβ1 group, TGFβ1 + GSK-J4 group were set up respectively, and scratch widths at 0 h, 24 h and 48 h were observed to evaluate cell migration. ( B , C ) Transwell migration experiment. The ability of the cells to pass to the lower chamber was observed. ( D-F ) RT-qPCR and western blot. The mRNA and protein levels of mmp2 /MMP2 and mmp9 /MMP9, which are related to cell migration and invasion were detected. Scale: 200 μm.(Data are means ± SEM from three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. * control vs. TGFβ1; # TGFβ1 vs. TGFβ1 + GSK-J4)

Article Snippet: GSK-J4 (T3100) and TGFβ1 (TMPY-02638) was purchased from Targetmol, Shanghai, China.

Techniques: Migration, Control, Quantitative RT-PCR, Western Blot

Journal: Medical Oncology (Northwood, London, England)

Article Title: Anti-tumor effects and mechanism of the histone demethylase inhibitor GSK-J4 in non-small cell lung cancer cells

doi: 10.1007/s12032-025-03185-3

Figure Lengend Snippet: GSK-J4 inhibits TGFβ1-induced EMT of A549 and H1299 cell lines. ( A ) RT-qPCR was used to detect EMT related markers, cdh1 , cdh2 , fn1 . ( B , C ) Western blot analysis was performed to detect the expression changes of E-cadherin, N-cadherin and FN1 at the protein levels.(Data are means ± SEM from three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. * control vs. TGFβ1; # TGFβ1 vs. TGFβ1 + GSK-J4)

Article Snippet: GSK-J4 (T3100) and TGFβ1 (TMPY-02638) was purchased from Targetmol, Shanghai, China.

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Control

Journal: Medical Oncology (Northwood, London, England)

Article Title: Anti-tumor effects and mechanism of the histone demethylase inhibitor GSK-J4 in non-small cell lung cancer cells

doi: 10.1007/s12032-025-03185-3

Figure Lengend Snippet: GSK-J4 inhibits TGFβ1-induced EMT of A549 and H1299 cell lines via the Wnt/β-catenin signaling pathway. ( A ) Changes of markers associated with the Wnt/β-catenin signaling pathway at mRNA levels. ( B, C ) Western blot was used to detect the changes of markers related to Wnt/β-catenin signaling pathway at the protein level. ( D )Expression and localization of β-catenin in A549 and H1299 cells induced by TGFβ1 by immunofluorescence staining. Scale bar : 100 μm. (Data are means ± SEM from three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. * control vs. TGFβ1; # TGFβ1 vs. TGFβ1 + GSK-J4)

Article Snippet: GSK-J4 (T3100) and TGFβ1 (TMPY-02638) was purchased from Targetmol, Shanghai, China.

Techniques: Western Blot, Expressing, Immunofluorescence, Staining, Control

Journal: Medical Oncology (Northwood, London, England)

Article Title: Anti-tumor effects and mechanism of the histone demethylase inhibitor GSK-J4 in non-small cell lung cancer cells

doi: 10.1007/s12032-025-03185-3

Figure Lengend Snippet: Restoration experiments were performed on Licl treated cells. ( A, B ) Transwell performed cell migration experiments. ( C–F ) Changes in the protein levels of MMP2 and MMP9, markers related to cell migration and invasion. ( G–J ) Western blot detection of changes in markers related to the Wnt/β-catenin signaling pathway at protein level. (Data are means ± SEM from three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. * control vs. TGFβ1;#TGFβ1 vs. TGFβ1 + GSK-J4; ^TGFβ1 + GSK-J4 vs. TGFβ1 + GSK-J4 + Licl)

Article Snippet: GSK-J4 (T3100) and TGFβ1 (TMPY-02638) was purchased from Targetmol, Shanghai, China.

Techniques: Migration, Western Blot, Control